Simple Spectrophotometric Method for Analysis of Serum Catalase Activity

A simple method for the determination of serum cholesterol

A simple spectrophotometric method for the determination of blood sugar by phenylhydrazine Hydrochlorlde

Simple Spectrophotometric Sequential Injection Analysis System for Determination of Serum Calcium

A sequential injection analysis (SIA) system for the determination of total calcium in blood has been implemented. The assay principle is based on the arsenazo III method, in which the indicator’s complex with calcium forms a blue-purple colour. The absorbance peak height at 650 nm was proportional to the calcium content in samples. A linear range was obtained from 2.5 15 mg·dL (r = 0.999), wit...

A continuous spectrophotometric method for measuring the activity of serum alkaline phosphatase.

A continuous spectrophotometric method for measuring serum alkaline phosphatase activity is described. The effects of temperature, pH, substrate concentration, type and molarity of the buffer, sample size, cofactors, and inhibitors on the enzymatic hydrolysis of p-nitrophenyl phosphate were studied. The optimal conditions for assay of serum alkaline phosphatase at 300 were found to be 0.75 M 2-...

A Simple Assay for Measuring Catalase Activity: A Visual Approach

In this study, an assay that combines the ease and simplicity of the qualitative approach for measuring catalase activity was developed. The assay reagents comprised only hydrogen peroxide and Triton X-100. The enzyme-generated oxygen bubbles trapped by Triton X-100 were visualized as foam, whose height was estimated. A calibration plot using the defined unit of catalase activity yielded the be...